Proteins of the Bacillus stearothermophilus ribosome. A low resolution crystal analysis of protein L30.
نویسندگان
چکیده
The 5 A resolution crystal structure analysis of ribosomal protein L30 from Bacillus stearothermophilus is described. The molecule is shown to be compact and extend to about 25-30 A in each dimension.
منابع مشابه
Proteins of the Bacillus stearothermophilus Ribosome
Proteins L30 and 55 from the 50 S and 30 S subunits, respectively, of the Bacillus stearothermophilus ribosome have been crystallized. L30 crystals are tetragonal and the space group is P41212 (or P43212)vwith cell dimensions a = b = 46.3 A and c = 61.4 A. S5 crystals are trigonal with the space group P3,21 (or P3,21) *and cell dimensions a = b = 59.3 A and c = 109.8 A. In both cases, there app...
متن کاملCloning, sequencing, and overexpression of genes for ribosomal proteins from Bacillus stearothermophilus.
Although a low resolution model for the arrangement of the proteins of the small and large ribosomal subunits is known, a detailed mechanistic understanding of the function of the ribosome awaits a high resolution structure of its components. While crystals have been obtained of several ribosomal proteins from Bacillus stearothermophilus, determination of atomic resolution structures of these p...
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Crystals of ribosomal protein L6 from Bacillus stearothermophilus suitable for high resolution structural studies have been obtained. Crystals are hexagonal with space group P6122 (or the enantiomorph P6522) and cell dimensions a = b = 72.7 A, c = 124.9 A. A search for heavy atom derivatives is in progress.
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Twelve of the proteins from the 30S ribosome of Bacillus stearothermophilus were isolated by preparative disc electrophoresis. Amino acid analyses of these proteins showed them to be different from each other. The gross amino acid composition of 30S ribosomal protein from B. stearothermophilus and Escherichia coli are virtually identical. A number of the proteins of B. stearothermophilus had el...
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ورودعنوان ژورنال:
- FEBS letters
دوره 160 1-2 شماره
صفحات -
تاریخ انتشار 1983